乙酰化EGCG的制备研究Study on Preparation of Acetylated EGCG
刘晓辉;江和源;张建勇;袁新跃;崔宏春;
摘要(Abstract):
通过对EGCG乙酰化衍生反应中条件的控制来合成全取代的乙酰化产物,反应产物再经初步纯化与柱层析再纯化2个过程制备得到了高纯度的乙酰化衍生物。
关键词(KeyWords): EGCG;乙酰化衍生物;纯化;制备
基金项目(Foundation): 浙江省科技计划(2007C22072)
作者(Author): 刘晓辉;江和源;张建勇;袁新跃;崔宏春;
Email:
DOI: 10.13989/j.cnki.0517-6611.2009.24.020
参考文献(References):
- [1]杨贤强,王岳飞,陈留记,等.茶多酚化学[M].上海:上海科学技术出版社,2003:109-386.
- [2]沈生荣,杨贤强,贾春友.油溶性茶多酚对色拉油的抗氧化作用[J].茶叶,1998,24(30):134-137.
- [3]SANGS,LAMBERTJ D,YANG C S.Reviewbioavailability and stabilityissues in understanding the cancer preventive effects of tea polyphenols[J].J Sci Food Agric,2006,86:2256-2265.
- [4]CHENL,LEE MJ,LI H,et al.Absorption,distribution,elimination of teapolyphenols in rats[J].Drug Metab Dispos,1997,25:1045-1050.
- [5]LAMBERTJ D,LEE MJ,LU H,et al.Epigallocatechin-3-gallate is ab-sorbed but extensively glucuronidated followingoral administration to mice[J].J Nutr,2003,133:4172-4177.
- [6]孙东,陈平.EGCG肉豆蔻酸酯的制备、结构及其抗氧化活性[J].温州医学院学报,2006,36(3):225-227.
- [7]TANAKAT,KUSSANOR,KOUNOI.Synthesis and antioxidant activity ofnovel amphipathic derivatives of tea polyphenol[J].Bioorga&Med ChemLett,1998(8):1801-1806.
- [8]YANOS,FUJIMURAY,UMEDAD,etal.Relationship betweenthe biolog-ical activities of methylated derivatives of(-)-epigallocatechin-3-O-gallate(EGCG)and theircell surface bindingactivities[J].J Agric Food Chem,2007,55:7144-7148.
- [9]MENG X,SANG S,ZHU N,et al.Identification and characterization ofmethylated and ring-fission metabolites oftea catechins formed in humans,mice and rats[J].ChemRes Toxicol,2002,15:1042-1050.
- [10]SAKAI M,SUZUKI M,NANJOF,et al.3-O-acylated catechins and meth-od of producing same:EP,0618203[P].1994.
- [11]沈生荣,金超芳,杨贤强.儿茶素的分子修饰[J].茶叶,1999,25(2):76-79.
- [12]MOONY H,LEE J H.Synthesis,structure analyses,and characterizationof novel epigallocatechin gallate(EGCG)glycosides using the glucansu-crase from Leuconostoc mesenteroides B-1299CB[J].J Agric FoodChem,2006,54:1230-1237.
- [13]SATOT,NAKAGAWAH.α-anomer-selective glucosylation of(+)-cate-chin by the crude enzyme,showing glucosyl transfer activity,of xan-thomonas campestris VW-9701[J].J Biosci Bioeng,2000,90:625-630.
- [14]LAMWH,KAZI A,HUHNDJ,etal.Apotential prodrugfora green teapolyphenol proteasome inhibitor:evalution of the peracetate eater of(-)-epigallocatechin-gallate[(-)-EGCG][J].Bioorga&Med Chem,2004,12:5587-5593.
- [15]LANDIS-PIWOWAR K,R,HUO C,CHEN D,et al.A Novel prodrug ofthe green tea polyphenol(-)-epigallocatechin-3-gallate as a potential an-ticancer agent[J].Cancer Res,2007,67(9):4303-4310.
- [16]KUHND J,LAMWH,KAZI A,et al.Synthetic peracetate tea polyphe-nols as potent proteasome inhibitors and apoptosis inducers in humancancer cells[J].Front Biosci,2005,10:1010-1023.
- [17]LAMBERTDJ,SANGS,HONGJ,etal.Peracetylation as a means of en-hancinginvitrobioactivityand bioavailabilityofepigallocatechin-3-gallate[J].Drug Metab Dispos,2006,34(12):2111-2116.