试验性趾叶炎小鼠肠组织中P物质的表达Expression of Substance P in Intestinal Tissue of Experimental Mice with Digital Lobule Inflammation
刘淑英;于洪川;王寒;
摘要(Abstract):
[目的]为动物趾叶炎的预防和治疗提供免疫学基础。[方法]对昆明种小白鼠注射二磷酸组织胺制备趾叶炎小鼠病理模型,以注射生理盐水为对照。分别于给药后2、14、24、48、72 h及15、30 d取小鼠3~5 mm长的小肠组织块,检测肠组织中炎性细胞、杯状细胞、肥大细胞及其脱颗粒数、P物质表达水平的动态变化。[结果]趾叶炎小鼠的肠腺结缔组织内及其绒毛上P物质表达异常丰富,且P物质呈单根或片状走行,并与肥大细胞串联及相邻排列,随给药时间的延长,P物质表达逐渐增强,肥大细胞及其脱颗粒数也急剧增多;炎性细胞、杯状细胞、腺体数量与肥大细胞的变化趋势一致;给药72 h后,血管内壁出现裂隙,肠粘膜出现水肿。[结论]肥大细胞参与小鼠趾叶炎的发病过程,并影响小鼠肠粘膜的通透性。
关键词(KeyWords): 趾叶炎;肥大细胞;P物质
基金项目(Foundation): 宁夏教育厅项目
作者(Author): 刘淑英;于洪川;王寒;
Email:
DOI: 10.13989/j.cnki.0517-6611.2009.16.128
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