付金颖;杨超;李德文;唐中华;郭晓瑞;祖元刚;

• 1:东北林业大学

摘要(Abstract)：

[目的]探讨环境紫外(UV-B)辐射对叶用杜仲叶片光合作用和活性物质含量及膜脂过氧化的影响,为叶用杜仲林的生态培育提供理论依据。[方法]在不同紫外(UV-B)辐射强度处理[CK:+0;T1:+1.40 kJ/(m~2·d);T2:+2.81 kJ/(m~2·d)]下,以5年生杜仲为研究对象,采用单因素方差分析与相关分析方法研究净光合速率、光合色素、次生代谢产物及自由基含量差异及相关性。[结果]UV-B辐射强度增加显著降低杜仲叶片光合参数净光合速率(Pn)、气孔导度(Gs)、胞间CO_2浓度(Ci)、蒸腾速率(Tr)和叶绿素a/b,显著提高了叶绿素a、叶绿素b、类胡萝卜素和总叶绿素含量,T1处理的叶绿素含量最高[(1.15±0.049)mg/g],T2处理的类胡萝卜含量最高[(0.036 0±0.001 6)mg/g];UV-B辐射强度增加显著降低杜仲叶片部分次生代谢产物桃叶珊瑚苷、京尼平苷酸、京尼平苷和绿原酸含量,且最大变化值之间分别相差20.74、61.23、16.16和13.63倍,同时也显著提高了部分次生代谢产物京尼平、黄酮、总酚和单宁含量,且T1处理的京尼平、黄酮、总酚和单宁含量分别是CK处理的1.21、1.11、1.34和1.37倍,总酚与单宁含量以T2处理最高,分别为(1.290 0±0.023 0)和(17.30±1.44)μg/g;UV-B辐射增强显著增加了MDA、H_2O_2和O_2·~-含量,且T1处理的MDA含量与O_2·~-含量较CK处理组分别高39.46%、70.48%;T2处理的H_2O_2含量较CK处理组高43.51%。[结论]UV-B辐射显著影响杜仲叶片光合参数及绿原酸、桃叶珊瑚苷、京尼平苷和京尼平苷酸等主要活性物质含量,因此可以通过调节环境UV-B辐射强度以提高叶用杜仲林的经济效益。

关键词(KeyWords)： 杜仲;UV-B辐射;净光合速率;茶多酚;绿原酸

Abstract:

Keywords:

基金项目(Foundation): 中央高校基本科研业务费用专项(2572015CA03);; 林业公益性行业科研专项(201504701-2);; 中央高校基本科研业务费专项(2572014EA01-02)

作者(Author): 付金颖;杨超;李德文;唐中华;郭晓瑞;祖元刚;

Email:

DOI: 10.13989/j.cnki.0517-6611.2017.26.002

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