杨梅;袁文杰;凤丽华;

• 1:吉林化工学院环境与生物工程学院
• 2:大连理工大学生物科学与技术学院
• 3:中国石油吉林石化公司炼油厂

摘要(Abstract)：

[目的]在联合生物加工技术发酵菊芋生料生成乙醇工艺中,降低高浓度菊芋生料醪液的黏度,使菊芋发酵乙醇浓度达到或超过目前玉米乙醇的行业水平。[方法]利用马克斯克鲁维酵母(Kluyveromyces marxianus),通过添加辅助酶制剂、改变拌料水温及优化补料方式等方法,降低发酵醪液黏度,提高菊芋生料的浓度,从而提高发酵终点的乙醇浓度并缩短发酵时间。[结果]最佳工艺条件为:45℃水拌料,pH 5.0,添加0.10%酒精复合酶,发酵初始干粉浓度为240 g/L,分别在发酵12和24 h补料,菊芋干粉终浓度可达到300 g/L,发酵时间48 h,乙醇浓度达到91.6 g/L,乙醇对糖的得率为0.464,为理论值的90.6%。[结论]此工艺为菊芋乙醇工业化的生产提供了有利条件。

关键词(KeyWords)： 菊芋;燃料乙醇;联合生物加工;马克斯克鲁维酵母;批式补料

Abstract:

Keywords:

基金项目(Foundation): 辽宁省基金项目(2008RC57)

作者(Author): 杨梅;袁文杰;凤丽华;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.09.081

参考文献(References)：

• [1]BACON J S D,EDELMAN J.The carbohydrates of the Jerusalem artichokeand other composite[J].Biochemical Journal,1951,48:114-126.
• [2]LYND L R,Van ZYL W H,MCBRIDE J E,et al.Consolidated bioprocess-ing of cellulosic biomass:An update[J].Curr Opin Biotechnol,2005,16(5):577-583.
• [3]徐丽丽,沈煜,鲍晓明.酿酒酵母纤维素乙醇统合加工(CBP)的策略及研究进展[J].生物工程学报,2010,26(7):870-879.
• [4]OHTA K.Production of high concentrations of ethanol from inulin by simu-taneous saccharification and fermentation using Aspergillus niger and Sac-charomyces cerevisiae[J].Applied and Environmental Microbiology,1993,59(3):729-733.
• [5]RONWENHORST R J,Van DER BAAN A A,SCHEFFERS W.Productionand localization ofβ-fructosidase in asynchronous and synchronous che-mostat cultures of yeasts[J].Appl Environ Microbio,1991,57(2):557-562.
• [6]RONWENHORST R J,RITMEESTER W S.Localization of inulinase andinvertase in Kluyveromyces specis[J].Appl Environ Microbio,1990,56(11):329-336.
• [7]袁文杰,任剑刚,赵心清,等.一步法发酵菊芋生产乙醇[J].生物工程学报,2008,24(11):1931-1936.
• [8]天津轻工业学院,大连轻工业学院,无锡轻工业学院.工业发酵分析[M].北京:中国轻工业出版社,1994:6-15.
• [9]MILLER G L.Use of dinitrosalicylic acid reagent for determination of re-ducing sugar[J].Anal Chem,1959,31:426-428.
• [10]XU T J,ZHAO X Q,BAI F W.Continuous ethanol production using self-flocculating yeast in a cascade of fermentors[J].Enzyme Microbial Tech-nol,2005,37(6):634-640.
• [11]李杰,辛本荣,隆小华,等.菊芋块茎的低温糊化及酸解条件的优化[J].食品工业科技,2010(3):239-242.
• [12]LI J J,TANG Y B,TANG X H,et al.Composite application of enzyme inthe production of fuel alcohol fermentation of Jerusalem artichoke flour[J].Liquor-making Science&Technology,2009,36:5-68.
• [13]BERLIN A,GILKES N,KILBURN D,et al.Evaluation of novel fungal cel-lulase preparations for ability to hydrolyze softwood substrates evidence forthe role of accessory enzymes[J].Enzyme Microb Technol,2005,37:175-184.
• [14]NAKAMURA T,OGATA Y,HAMADA S,et al.Ethanol production fromJerusalem artichoke tubers by Aspergillus niger and Saccharomyces cerevi-siae[J].Journal of Fermentation and Bioengineering,1996,81:564-566.
• [15]GE X Y,ZHANG W G.A shortcut to the production of high ethanol con-centration from Jerusalem artichoke tuber[J].Food Technol Biotechnol,2005,43(3):241-246.
• [16]俞静,江佳稀,张永强,等.鹰嘴豆孢克鲁维酵母利用菊芋原料同步糖化与发酵生产乙醇[J].生物工程学报,2010,26(7):982-990.