菊芋生料联合生物加工发酵生产燃料乙醇Production of Fuel Ethanol from Uncooked Helianthus tuberosus by Consolidated Bio-processing(CBP) Fermentation
杨梅;袁文杰;凤丽华;
摘要(Abstract):
[目的]在联合生物加工技术发酵菊芋生料生成乙醇工艺中,降低高浓度菊芋生料醪液的黏度,使菊芋发酵乙醇浓度达到或超过目前玉米乙醇的行业水平。[方法]利用马克斯克鲁维酵母(Kluyveromyces marxianus),通过添加辅助酶制剂、改变拌料水温及优化补料方式等方法,降低发酵醪液黏度,提高菊芋生料的浓度,从而提高发酵终点的乙醇浓度并缩短发酵时间。[结果]最佳工艺条件为:45℃水拌料,pH 5.0,添加0.10%酒精复合酶,发酵初始干粉浓度为240 g/L,分别在发酵12和24 h补料,菊芋干粉终浓度可达到300 g/L,发酵时间48 h,乙醇浓度达到91.6 g/L,乙醇对糖的得率为0.464,为理论值的90.6%。[结论]此工艺为菊芋乙醇工业化的生产提供了有利条件。
关键词(KeyWords): 菊芋;燃料乙醇;联合生物加工;马克斯克鲁维酵母;批式补料
基金项目(Foundation): 辽宁省基金项目(2008RC57)
作者(Author): 杨梅;袁文杰;凤丽华;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.09.081
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