凌瑞;孙立洁;陈祥松;袁丽霞;吴金勇;潘淼;姚建铭;

• 1:中国科学院合肥物质科学研究院等离子体物理研究所
• 2:中国科学院湖北产业技术创新与育成中心

摘要(Abstract)：

花生四烯酸(Arachidonic acid)是ω-6系列的长链多不饱和脂肪酸,对人体心血管系统、免疫系统及神经发育等有多重调节作用。近年来,随着花生四烯酸在食品、化妆品和医药等领域的广泛应用及研究的不断深入,以高山被孢霉发酵法产花生四烯酸在育种和发酵调控等方面取得了较大进展。对国内外在该领域的研究进展进行了综述,并对其研究前景进行了展望。

关键词(KeyWords)： 花生四烯酸;育种;发酵;代谢调控

Abstract:

Keywords:

基金项目(Foundation): 国家高技术研究发展计划项目(2014AA021703);; 教育部留学回国人员科研启动基金资助项目(教外司留[2013]693号)

作者(Author): 凌瑞;孙立洁;陈祥松;袁丽霞;吴金勇;潘淼;姚建铭;

Email:

DOI: 10.13989/j.cnki.0517-6611.2015.33.036

参考文献(References)：

• [1]TOTANI N,OBA K.The filamentous fungusmortierellaalpina,high in arachidonic acid[J].Lipids,1987,22(12):1060-1062.
• [2]王安琪,戴传超,赵沫,等.轮梗霉高产花生四烯酸的诱变育种研究[J].食品科学,2007(2):158-162.
• [3]贾红华,周华,韦萍.微波诱变育种研究及应用进展[J].工业微生物,2003,33(2):46-50.
• [4]李丽娜,汤华成,于长青.深黄被孢霉高产花生四烯酸菌株的微波育种[J].食品与生物技术学报,2009(1):117-121.
• [5]周蓬蓬,余龙江,汪建华,等.微波等离子体溅射诱变选育花生四烯酸高产菌及补料工艺研究[J].激光生物学报,2003,12(1):59-62.
• [6]姚建铭,王纪,王相勤,等.离子注入花生四烯酸产生菌诱变选育[J].生物工程学报,2000(4):478-481.
• [7]袁成凌,姚建铭,王纪,等.低能离子注入在花生四烯酸(AA)高产菌株选育中的研究[J].辐射研究与辐射工艺学报,2003,21(4):237-242.
• [8]余增亮,王纪,袁成凌,等.微生物油脂花生四烯酸产生菌离子束诱变和发酵调控[J].科学通报,2012,57(11):883-890.
• [9]吴定,路桂红.低能离子注入法诱变微生物育种[J].中国酿造,2002,21(S1):32.
• [10]杨立峰.低能离子注入大肠杆菌的诱变选育及培养优化[D].南京:南京工业大学,2005.
• [11]吕杰,金湘,毛培宏.离子束注入诱变技术应用于食用菌的研究[J].中国食用菌,2011,30(5):3-5.
• [12]王姗杰,詹晓北,吴剑荣,等.低能氮离子注入诱变选育高产结冷胶菌株的研究[J].工业微生物,2010,40(2):20-24.
• [13]WANG W X,ZHAO S L,SHE J,et al.Breeding a mortierella alpina saltant of high ARA production[J].Journal of the Chinese cereals&oils association,2014,29(7):92-95.
• [14]马瑞雪,何东平,陈涛.Co60辐射诱变深黄被孢霉高产多不饱和脂肪酸突变株的选育[J].中国油脂,2004,29(12):48-50.
• [15]周蓬蓬,余龙江,朱敏.花生四烯酸产生菌的原生质体诱变育种[J].华中理工大学学报,2000,28(7):105-106.
• [16]SAKURADANI E,HIRANO Y,KAMADA N,et al.Improvement of arachidonic acid production by mutants withlower n-3 desaturation activity derived from Mortierella alpine 1S-4[J].Appl Microbial Biotechnol,2004,66:243-248.
• [17]王婷婷,詹晓北,郑志永,等.高产花生四烯酸高山被孢霉菌株的诱变筛选[J].工业微生物,2013,43(4):44-50.
• [18]于长青,李丽娜.深黄被孢霉高产花生四烯酸菌株的紫外诱变原生质体育种[J].微生物学报,2009,49(1):44-48.
• [19]赵沫,戴传超,王安琪,等.高产花生四烯酸菌的原生质体诱变育种研究[J].食品科学,2008,29(2):275-279.
• [20]WANG L,CHEN W,FENG Y,et al.Genome characterization of the oleaginous fungus mortierell aalpina[J].Plos One,2011,6(12):28319.
• [21]SAKURADANI E,KIKUKAWA H,TAKENO S,et al.Transformation of zygomycete Mortierella alpina using biolistic particle bombardment[J].Genetic transformation systems in fungi,2015,1:135-140.
• [22]ZHANG C J,LING X P,ZENG S Y,et al.Study on protoplast transformation of Mortierella alpina and its homologous expression of elongase gene[J].Journal of Xiamen University,2012,51(5):911-917.
• [23]TAKENO S,SAKURADANI E,TOMI A,et al.Transformation of oilproducing fungus,mortierellaalpina 1S-4,using zeocin,and application to arachidonic acid production[J].Journal of bioscience and bioengineering 2005,100(6):617-622.
• [24]落合美佐,河岛洋,清水昌.被孢霉属(Mortierella)脂质生产菌的育种方法:日本,200480023946[P].2008-04-16.
• [25]DAI M H,ZIESMAN S,RATCLIFFE T,et al.Visualization of protoplast fusion and quantitation of recombination in fused protoplasts of auxotrophic strains of Escherichia coli[J].Metabolic engineering,2005,7(1):45-52.
• [26]TIAN J,JI H,OKU H,et al.Effects of dietary arachidonic acid(ARA)on lipid metabolism and health status of juvenile grass carp,Ctenopharyngodon idellus[J].Aquaculture,2014,430(15):57-65.
• [27]刘欣.花生四烯酸生产菌高山被孢霉的代谢组学研究[D].南京:南京工业大学,2012.
• [28]JANG H D,LIN Y Y,YANG S S.Effect of culture media and conditions on polyunsaturated fatty acids production by Mortierella alpina[J].Bioresource technology,2005,96(15):1633-1644.
• [29]MELO I S,SANTOS S N,ROSA L H,et al.Isolation and biological activities of an endophytic Mortierella alpina strain from the Antarctic moss Schistidium antarctici[J].Extremophiles,2014,18(1):15-23.
• [30]WU W J,YAN J C,JI X,et al.Lipid characterization of an arachidonic acid-rich oil producing fungus Mortierella alpina[J].Chinese journal of chemical engineering,2015,23(7):1183-1187.
• [31]GAO M J,WANG C,ZHENG Z Y,et al.Study on improved arachidonic acid production by mortierella alpina with dynamic temperature-control in airlift bioreactor[J].Science&technology of food industry,2014,35(22):179-183.
• [32]焦敏,张湜,李丽娟,等.花生四烯酸发酵过程的建模方法研究[J].自动化仪表,2011,32(11):29-31.
• [33]PENG C,HUANG H,JI X,et al.A temperature-shift strategy for efficient arachidonic acid fermentation by Mortierella alpina in batch culture[J].Biochemical engineering journal,2010,53(1):92-96.
• [34]PENG C,HUANG H,JI X,et al.Effects of n-hexadecane concentration and a two-stage oxygen supply control strategy on arachidonic acid production by Mortierella alpina ME-1[J].Chemical engineering&technology,2010,33(4):692-697.
• [35]JIN M J,HE H,XIAO A H,et al.A novel two-step fermentation process for improved arachidonic acid production by Mortierella alpina[J].Biotechnology letters,2008,30(6):1087-1091.
• [36]WANG H H,ISAACS F J,CARR P A,et al.Programming cells by multiplex genome engineering and accelerated evolution[J].Nature,2009,460(7257):894-898.
• [37]张立伟.全局转录工程选育耐乳酸鼠李糖乳酸杆菌[D].武汉:华中科技大学,2013.