段新平;高飞;武蓉;宋奇;韩杨;姚潮刚;纪元;

• 1:吉林大学畜牧兽医学院动物胚胎工程吉林省重点实验室

摘要(Abstract)：

[目的]建立PIWIL1基因启动子调控的特异性表达Cre重组酶的转基因猪,为研究piRNA及其相关基因的功能和机制奠定基础。[方法]通过PCR扩增,从猪的全基因组上扩增出PIWIL1基因的启动子。切除猪源Cre重组酶的表达载体pET28a-MHC-Cre-BGHpo1yA-FRT2neor的启动子MHC,将其与PIWIL1基因启动子连接,构建成特异性表达Cre重组酶的pPIWIL1-Cre载体。将该载体转染小型猪胎儿成纤维细胞,通过G418筛选,获得阳性克隆进行核移植。[结果]获得2头PIWIL1基因启动子调控的特异表达Cre酶的转基因猪。[结论]成功构建了PIWIL1基因启动子调控的特异表达Cre重组酶的转基因猪,为研究piRNA及其相关基因的功能和机制提供了工具猪。

关键词(KeyWords)： piRNA;PIWIL1;Cre酶;转基因猪

Abstract:

Keywords:

基金项目(Foundation): 国家重点基础研究发展计划(973计划)资助(2011CB944203)

作者(Author): 段新平;高飞;武蓉;宋奇;韩杨;姚潮刚;纪元;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.09.216

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