鸡源大肠杆菌中QRDR基因的检测Detection of QRDR Gene in Escherichia coli from Chicken
冯涛;何纪元;薛原;
摘要(Abstract)：
为分析鸡源大肠杆菌对喹诺酮类药物的耐药机制,使用PCR技术从对喹诺酮类药物具有耐药性的大肠杆菌中扩增出喹诺酮耐药决定区(QRDR)基因。通过对所测序列结果的分析,得到DNA促旋酶和拓扑异构酶Ⅳ基因(gyrA、gyrB、parC、parE)的突变位点及其氨基酸的表达情况。根据基因型研究QRDR的流行病学情况,可指导喹诺酮类药物的临床合理用药。
关键词(KeyWords)： 大肠杆菌;gyrA基因;gyrB基因;parC基因;parE基因
基金项目(Foundation): 国家自然科学基金项目(31502119);; 黑龙江省博士后科研启动金资助项目(LBH-Q14002)
作者(Author): 冯涛;何纪元;薛原;
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DOI:
参考文献(References)：
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