刘超頔;侯占铭;

• 1:内蒙古师范大学生命科学与技术学院

摘要(Abstract)：

[目的]敲除小麦赤霉菌(Fusarium graminearum)FGSG_03700基因,确定其缺失突变体表型,从而分析该基因的生物学功能。[方法]应用Split Marker基因敲除技术,构建含有潮霉素抗性基因hph的基因敲除盒,通过PEG介导,进行原生质体转化,在含有潮霉素的培养基上筛选转化子。[结果]通过PCR正负筛查,得到3个确定的突变体,分别命名为ΔFGSG_03700 1-2、ΔFGSG_03700 2-2、ΔFGSG_03700 2-3。通过表型验证及孢子对番茄侵染试验发现,敲除突变体的菌落形态及生长速度没有明显变化,致病力没有减弱,但突变体的产孢量低于野生型PH-1。[结论]FGSG_03700基因可能与小麦赤霉菌分生孢子的生长能力有关。

关键词(KeyWords)： 小麦赤霉菌;Split Marker;基因敲除;FGSG_03700

Abstract:

Keywords:

基金项目(Foundation): 内蒙古自治区自然科学基金项目(2015MS0311);; 国家自然科学基金项目(31160280)

作者(Author): 刘超頔;侯占铭;

Email:

DOI: 10.13989/j.cnki.0517-6611.2017.04.047

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