杨宁;蓝东明;王永华;杨博;

• 1:华南理工大学生物科学与工程学院
• 2:华南理工大学食品科学与工程学院

摘要(Abstract)：

[目的]研究新的脂肪酶及优化脂肪酶发酵培养条件。[方法]在毕赤酵母X33系统中重组表达白假丝酵母脂肪酶CaLIP5。利用响应面分析法,构建了CaLIP5的酶活回归方程。[结果]由响应面分析可知,在温度25.71℃,pH8.33,酵母提取物浓度1.28%和葡萄糖浓度4.06%时,预测最大酶活为8.33U/ml。试验验证酶活为9.24U/ml。实际试验值与模型预测值基本一致。优化后的酶活提高了22.22%。[结论]在优化的培养条件下,可以获得毕赤酵母X33重组表达CaLIP5的最大酶活。

关键词(KeyWords)： 白假丝酵母脂肪酶;重组表达;优化培养基;响应面法

Abstract:

Keywords:

基金项目(Foundation): 广州市科技攻关项目(2009A1-E)

作者(Author): 杨宁;蓝东明;王永华;杨博;

Email:

DOI: 10.13989/j.cnki.0517-6611.2011.17.044

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