朱占东;刘学锋;柴守玺;

• 1:甘肃农业大学农学院
• 2:新疆生产兵团第十四师一牧场

摘要(Abstract)：

[目的]探讨小麦Ta NRX基因与抗旱的关系及其调控机制。[方法]根据晋麦Ta NRX基因序列,设计一对特异引物,利用RTPCR法从"平凉45号"冬小麦的c DNA中扩增出一个DNA片段,并克隆到p MD19-T载体,测序后比对分析。[结果]该片段具有完整的开放阅读框,长度为1 734 bp,编码577个氨基酸,与Genbank中晋麦的核酸序列具有100%的同源性,氨基酸序列中包含2个氧化还原二硫化物活性中心WCGPC和WCPPC,属于TRX家族,同时生物信息学分析发现该基因编码不稳定、非分泌、脂溶性蛋白。实时荧光定量PCR检测显示,该基因在"平凉45号"冬小麦各器官中均有表达,但具有不同的表达模式。[结论]为进一步深入研究Ta NRX基因的功能及与其他基因之间的相互关系提供参考。

关键词(KeyWords)： 小麦;TaNRX基因;克隆;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 朱占东;刘学锋;柴守玺;

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参考文献(References)：

• [1]ARNR E S J,HOLMGREN A.Physiological functions of thioredoxinand thioredoxin reductase[J].Eur J Biochem,2001,267:6102-6109.
• [2]WONG J H,BALMER Y,CAI N,et al.Unraveling thioredoxin-linked metabolicprocesses of cereal starchy endosperm using proteomics[J].FEBS Lett,2003,547:151-156.
• [3]MEYER Y,VIGNOLS F,REICHHELD J P.Classification of plant thioredoxins by sequence similarity and intron position[J].Methods Enzymol,2002,347:394-402.
• [4]张帆,蒋雷,鞠丽萍,等.一个普通小麦Trx超家族新基因Ta NRX的克隆与抗旱相关标记开发[J].作物学报,2014,40(1):29-36.
• [5]郑琼,马旭俊,杨传平.硫氧还蛋白(Trx)的研究进展[J].分子植物育种,2006,4(S2):78-82.
• [6]LAUGHNER B J,SEHNKE P C,FERL R J.A novel nuclear member ofthe thioredoxin superfamily[J].Plant Physiol,1998,118:987-996.
• [7]夏德习,管清杰,金淑梅,等.拟南芥硫氧还蛋白M1型基因(At TRX m1)与环境逆境之间的关系[J].分子植物育种,2007(5):21-26.
• [8]BROIN M,REY P.Potato plants lacking the CDSP32 plastidic thio-redoxin exhibit overoxidation of the BAS1 2-cysteine peroxire-doxin and increased lipid peroxidation in thylakoids underphotooxidative stress[J].Plant Physiol,2003,132:1335-1343.
• [9]魏邦龙,白天志,包世堂.甘肃小麦栽培模拟优化决策系统的研制[J].西北师范大学学报,2004,40(2):86-89.
• [10]任根深,王伟.冬小麦新品种平凉45号选育报告[J].甘肃农业科技,2010(5):3-5.
• [11]ZHENG W J,XU Z S,CHEN M,et al.Isolationand characterization of receptor-like protein kinase WELP1 inwheat[J].Afr J Microbiol Res,2012,6:2410-2418.
• [12]黄雪玲,冯浩,康振生.小麦条锈菌实时荧光定量PCR分析中内参基因的选择[J].农业生物技术学报,2012,20(2):181-187.
• [13]LIVAK K J,SCHMITTGEN T D.Analysis of relativegene expression data using real-time quantitative PCRa nd the 2-ΔΔCtmethod[J].Methods,2001,25:402-408.
• [14]KUROOKA H,KATO K,MINOGUCHI S,et al.Cloning and characterization of the nucleoredoxin gene that en-codes a novel nuclear protein related to thioredoxin[J].Genomics,1997,39:331-339.
• [15]卫丽,黄晓书,李鹏坤,等.植物硫氧还蛋白研究进展[J].贵州农业科学,2006,34(6):129-131.
• [16]GELHAYE E,ROUHIER N,NAVROT N,et al.The plant thioredoxin system[J].Cell Mol Life Sci,2005,62(1):24-35.
• [17]LAUGHNER B J,SEHNKE P C,FERL R J.A novel nuclear member of the thioredoxin superfamily[J].Plant Physiol,1998,118:987-996.
• [18]KUROOKA H,KATO K,MINOGUCHI S,et al.Cloning and characterization of the nucleoredoxin gene that en-codes a novel nuclear protein related to thioredoxin[J].Genomics,1997,39:331-339.
• [19]刘雷,尹钧.硫氧还蛋白的研究[J].东北农业大学学报,2003,34(2):219-225.
• [20]JUTTNER J,OLDE D,LANGRIDGE P,et al.Cloning and expression of a distinct subclass of plant thiore-doxins[J].Eur J Biochem,2000,267:7109-7117.