朱占东;刘学锋;柴守玺;

• 1:甘肃农业大学农学院
• 2:新疆生产兵团第十四师一牧场

摘要(Abstract)：

[目的]探讨小麦Ta NRX基因与抗旱的关系及其调控机制。[方法]根据晋麦Ta NRX基因序列,设计一对特异引物,利用RTPCR法从"平凉45号"冬小麦的c DNA中扩增出一个DNA片段,并克隆到p MD19-T载体,测序后比对分析。[结果]该片段具有完整的开放阅读框,长度为1 734 bp,编码577个氨基酸,与Genbank中晋麦的核酸序列具有100%的同源性,氨基酸序列中包含2个氧化还原二硫化物活性中心WCGPC和WCPPC,属于TRX家族,同时生物信息学分析发现该基因编码不稳定、非分泌、脂溶性蛋白。实时荧光定量PCR检测显示,该基因在"平凉45号"冬小麦各器官中均有表达,但具有不同的表达模式。[结论]为进一步深入研究Ta NRX基因的功能及与其他基因之间的相互关系提供参考。

关键词(KeyWords)： 小麦;TaNRX基因;克隆;实时荧光定量PCR

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 朱占东;刘学锋;柴守玺;

Email:

DOI: 10.13989/j.cnki.0517-6611.2015.18.012

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