胰蛋白酶浓度对H9亚型禽流感病毒在MDCK细胞上增殖的影响Effects of Trypsin on Proliferation of Avian Influenza Virus H9N2 Subtype in MDCK Cell
史爱华;姜北宇;沈佳;景小冬;章振华;李林;张建伟;
摘要(Abstract):
[目的]探讨不同胰蛋白酶浓度对低致病力禽流感病毒在MDCK细胞上增殖后的HA滴度。[方法]通过3株禽流感H9亚型病毒分别接种MDCK细胞单层,加入含有不同胰蛋白酶浓度的DMEM维持液,每隔24 h观察细胞病变,并测定上清液中的HA滴度。[结果]当维持液中胰蛋白酶含量为10~20μg/ml时,培养液上清液中的HA滴度最高,达到7 log2(1∶128)。当病毒的接种浓度为10-3和10-4时,MDCK细胞在96 h几乎全部病变,峰值出现在接种后的72~96 h。[结论]当维持液中胰蛋白酶含量为10~20μg/ml时,有利于H9亚型AIV病毒在MDCK细胞上增殖。
关键词(KeyWords): H9N2亚型禽流感病毒;MDCK细胞;胰蛋白酶浓度
基金项目(Foundation): 北京市农林科学院资助项目(2010A007)
作者(Author): 史爱华;姜北宇;沈佳;景小冬;章振华;李林;张建伟;
Email:
DOI: 10.13989/j.cnki.0517-6611.2011.35.223
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