宋娇;杨烨;喻小琼;刘冉冉;赵桂苹;

• 1:长江大学动物科学学院
• 2:中国农业科学院北京畜牧兽医研究所动物营养学国家重点实验室

摘要(Abstract)：

[目的]建立肌卫星细胞和肌内前体脂肪细胞的共培养体系。[方法]用10μg/ml INS、1μmol/L DEX和115 ng/ml IBMX对北京油鸡肌肉SV细胞进行诱导后,通过油红染色法、细胞免疫学鉴定及关键基因的表达对诱导的SV细胞进行分析。[结果]油红O脂肪染色法鉴定结果表明,诱导后的细胞红色脂质密度较高,说明SV细胞经过诱导后含有更多的前体脂肪细胞。通过实时荧光PCR定量检测细胞发育的标志性基因,发现诱导与未诱导的肌肉SV细胞均会表达前体脂肪细胞标志性基因。[结论]肌肉SV细胞经过诱导后前体脂肪细胞的含量得到提高,有利于建立前体脂肪细胞和肌卫星细胞的体外培养体系。

关键词(KeyWords)： 北京油鸡;前体脂肪细胞;肌卫星细胞;共培养体系;诱导

Abstract:

Keywords:

基金项目(Foundation): 湖北省自然科学基金项目(2011CDB012,2013CFA100)

作者(Author): 宋娇;杨烨;喻小琼;刘冉冉;赵桂苹;

Email:

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