北京油鸡骨骼肌SV细胞成脂诱导分化的研究Study on the Adipogenic Differentiation of SV Cells in Skeletal Muscles of Beijing You Chicken
宋娇;杨烨;喻小琼;刘冉冉;赵桂苹;
摘要(Abstract)：
[目的]建立肌卫星细胞和肌内前体脂肪细胞的共培养体系。[方法]用10μg/ml INS、1μmol/L DEX和115 ng/ml IBMX对北京油鸡肌肉SV细胞进行诱导后,通过油红染色法、细胞免疫学鉴定及关键基因的表达对诱导的SV细胞进行分析。[结果]油红O脂肪染色法鉴定结果表明,诱导后的细胞红色脂质密度较高,说明SV细胞经过诱导后含有更多的前体脂肪细胞。通过实时荧光PCR定量检测细胞发育的标志性基因,发现诱导与未诱导的肌肉SV细胞均会表达前体脂肪细胞标志性基因。[结论]肌肉SV细胞经过诱导后前体脂肪细胞的含量得到提高,有利于建立前体脂肪细胞和肌卫星细胞的体外培养体系。
关键词(KeyWords)： 北京油鸡;前体脂肪细胞;肌卫星细胞;共培养体系;诱导
基金项目(Foundation): 湖北省自然科学基金项目(2011CDB012,2013CFA100)
作者(Author): 宋娇;杨烨;喻小琼;刘冉冉;赵桂苹;
Email:
DOI: 10.13989/j.cnki.0517-6611.2015.15.055
参考文献(References)：
- [1]VETTOR R,MILAN G,FRANZIN C,et al.The origin of intermuscular adipose tissue and its pathophysiological implications[J].Am J Physiol Endocrinol Metab,2009,297:987-998.
- [2]SELL H,DIETZE-SCHROEDER D,ECKEL J.The adipocyte-myocyte axis in insulin resistance[J].TRENDS in Endocrinology and Metabolism,2006,17(10):416-422.
- [3]VU V,KIM W,FANG X,et al.Coculture with primary visceral rat adipocytes from control but not streptozotocin-induced diabetic animals increases glucose uptake in rat skeletal muscle cells:Role of adiponectin[J].Endocrinology,2007,148:4411-4419.
- [4]HAUSMAN G J,POULOS S P.A method to establish co-cultures of myotubes and preadipocytes from collagenase digested neonatal pig semitendinosus muscles[J].J Anim Sci,2005,83:1010-1016.
- [5]QUINN L S,ANDERSON B G,STRAIT-BODEY L,et al.Oversecretion of interleukin-15 from skeletal muscle reduces adiposity[J].Am J Physiol Endocrinol Metab,2009,296:191-202.
- [6]LIVAK K J,SCHMITTGEN T D.Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCTmethod[J].Methods,2001,25:402-408.
- [7]POULOS S P,DODSON M V,HOUSMAN G J.Cell line models for differentiation:preadipocytes and adipocytes[J].Experimental Biology and Medicine,2010,235:1185-1193.
- [8]TONTONOZ1 P,SPIEGELMAN B M.Fat and beyond:The diverse biology of PPARγ[J].Annu Rev Biochem,2008,77:289-312.
- [9]LEFTEROVA M I,LAZAR MA.New developments in adipogenesis[J].Trends Endocrinol Metab,2009,20:107-114.