植物HSP70反义基因工程雄性不育表达载体的构建及鉴定Construction and Identification of HSP70 Antisense RNA Expression Vector for Genetic Engineering Male Sterility in Plant
刘立科;刘根齐;侯宁;刘春光;陈建南;张文会;樊颖伦;吴冰洁;
摘要(Abstract)：
[目的]为更好地研究HSP70热激蛋白基因与植物雄性不育的关系。用PCR方法克隆了水稻花药特异表达启动子Osg6B,将其与设计合成的HSP70反义片段连接,构建了由水稻花药特异表达启动子Osg6B驱动的HSP70反义表达载体,并进行了PCR和酶切鉴定。[结果]克隆的Osg6B启动子序列与所发表的序列同源性高达97%,启动子区域的顺式调控元件完整;构建的由水稻花药特异表达启动子Osg6B驱动的HPS70反义表达载体通过了菌落PCR验证和表达载体值粒的酶切鉴定,载体构建成功。[结论]该表达载体的构建将为植物基因工程雄性不育的利用奠定基础。
关键词(KeyWords)： HSP70;Osg6B启动子;基因工程雄性不育
基金项目(Foundation): 国家自然基金(30400283)
作者(Author): 刘立科;刘根齐;侯宁;刘春光;陈建南;张文会;樊颖伦;吴冰洁;
Email:
DOI: 10.13989/j.cnki.0517-6611.2008.16.012
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