磺胺间二甲氧嘧啶人工抗原的合成与鉴定Synthesis and Identification of the Sulfadimethoxine College Artificial Antigen
鲁晓翠;朱金凤;张改平;邓瑞广;侯玉泽;王自良;彩鸿翔;王青;
摘要(Abstract)：
用重氮化方法将SDM偶联于载体蛋白BSA和OVA上,合成免疫抗原BSA-SDM和包被抗原OVA-SDM,通过紫外扫描(UV)、SDS-PAGE凝胶电泳试验。结果显示:BSA-SDM和OVA-SDM的UV与BSA、SDM的相比均发生了改变,出现了明显的特征峰。这表明半抗原和载体偶联成功。用BSA-SDM免疫BALB/C小鼠,间接ELISA测定多抗上清(pAb)效价。结果表明,获得了高效价的pAb,为SDM残留免疫学检测方法的建立奠定了基础。
关键词(KeyWords)： 磺胺间二甲氧嘧啶;人工抗原;合成;鉴定
基金项目(Foundation): “十五”国家重大科技专项(2001BA804A30-08)
作者(Author): 鲁晓翠;朱金凤;张改平;邓瑞广;侯玉泽;王自良;彩鸿翔;王青;
Email:
DOI: 10.13989/j.cnki.0517-6611.2007.15.077
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