融合蛋白VP4-STI的免疫效果观察Observation of the Immune Effect of Fusion Protein VP4-STI
武军元;陈创夫;王智超;李有文;
摘要(Abstract)：
为了研究融合蛋白VP4-STI的免疫效果。将40只小鼠随机分为实验疫苗组(30μg VP4-STI+0.6μg LTB)、铝胶疫苗组[30μg VP4-STI+Al(OH)3胶]、纯化蛋白VP4-STI疫苗组(30μg VP4-STI)和PBS对照组,通过鼻腔滴入进行免疫,测定其抗体水平。用大肠杆菌强毒株C83902进行攻毒试验,观测各免疫组小鼠的保护效果。除PBS对照组外,其余各组均有抗VP4-STI抗体产生,第6周最高,铝胶疫苗组的抗体水平略高于实验疫苗组,纯化蛋白VP4-STI免疫组较低,与实验疫苗组差异极显著(P<0.001)。实验疫苗组和铝胶疫苗组小鼠对大肠杆菌强毒株C83902均有较好的免疫保护效果,与对照PBS组有明显差异。该研究为进一步提高STI的免疫原性提供了依据。
关键词(KeyWords)： 大肠杆菌;VP4;STI;疫苗
基金项目(Foundation): 新疆生产建设兵团博士基金(兵博02,NKBOISHZO8)
作者(Author): 武军元;陈创夫;王智超;李有文;
Email:
DOI: 10.13989/j.cnki.0517-6611.2007.21.101
参考文献(References)：
- [1]SVENERHOLM A M,WIKLUND G.Rapid GM1-enzym-linked immunosorbentassay with visual reading for identification of Escherichia coli heat-labile entero-toxin[J].Clin Microbiol,1983,17:596-600.
- [2]JERTBORNM,ARINGHREN C,HOLMGREN J,et al.Safety and immunogenic-ity of an oral inactivated enterotoxigenic Escherichia coli[J].Vaccine,1998,16:255-260.
- [3]GUZMAN VLM.Export and processing analysis of a fusion between theentrocelllμar heat-stable emterotoxin and the periplasmic B-subunit of the heat-labile enterotoxin inE.coli[J].Mol Microbiol,1990,4(2):253-264.
- [4]LAWRENCE R M,HUANG P T,GLICK J,et al.Expression of the cloned genefor enterotoxin STb ofEscherichia coli[J].Infect Immun,1990,58(4):970-977.
- [5]许崇波,冯书章,刘子,等.大肠杆菌耐热性肠毒素基因核苷酸序列分析与免疫原性研究[J].中国兽医学报,1996,16(4):327-332.
- [6]BISHOP RF.Development of candidate rotavirus vaccine[J].Vaccine,1993,11:247-254.
- [7]GREENBERY H B,VALDESUSO J,VAN WYKE K,et al.Production and pre-liminary characterization of monoclonal antibodies directed at two surface proteinof Rhesus rotavirus[J].Virol,1983,47:267-275.
- [8]孙茂盛.重组杆状病毒表达轮状病毒SAll毒株VP4蛋白[J].中国医学科学院学报,1997,19(1):48-53.
- [9]陈元鼎,刘名英,赵玮,等.重组轮状病毒SAll VP4抗原表位诱导广泛交叉中和抗体反应[J].中国病毒学,1998,13(1):57-63.
- [10]王鹏雁,陈创夫,余兴龙,等.轮状病毒VP4蛋白与肠产毒性大肠杆菌热稳定肠毒素蛋白的融合表达[J].中国人兽共患病杂志,2003,19(1):76-81.
- [11]KLIPSTEIN F A.Development of a vaccine of crosslinked heat-stable and heat-labile enterotoxins that protects againstE.coliproducing either enterotoxin[J].Infect Immun,1982,37:550-557.
- [12]CLEMENTS J D.Construction of a nontoxic fusion peptide for immunization a-gainstEscherichia colistrains that produce heat-stable and heat-labile entero-toxins[J].Infect Immun,1990,58:1159-1166.
- [13]HOLMGREN J,CZERKINSKY C,ERIKSSON K,et al.Mucosal immunizationand adjuvants:a brief overview of recent advances and challenges[J].Vaccine,2003,21(Suppl 2):89-95.
- [14]MILLAR D G,HIRSTTR,SNIDER D P.Escherichia coli heat-labile enterotox-in B subunit is a more potent mucosal adjuvant than its closely related homo-logue,the B subunit of cholera toxin[J].Infect Immun,2001,69(5):3476-3482.