融合蛋白VP4-STI的免疫效果观察Observation of the Immune Effect of Fusion Protein VP4-STI
武军元;陈创夫;王智超;李有文;
摘要(Abstract)：
为了研究融合蛋白VP4-STI的免疫效果。将40只小鼠随机分为实验疫苗组(30μg VP4-STI+0.6μg LTB)、铝胶疫苗组[30μg VP4-STI+Al(OH)3胶]、纯化蛋白VP4-STI疫苗组(30μg VP4-STI)和PBS对照组,通过鼻腔滴入进行免疫,测定其抗体水平。用大肠杆菌强毒株C83902进行攻毒试验,观测各免疫组小鼠的保护效果。除PBS对照组外,其余各组均有抗VP4-STI抗体产生,第6周最高,铝胶疫苗组的抗体水平略高于实验疫苗组,纯化蛋白VP4-STI免疫组较低,与实验疫苗组差异极显著(P<0.001)。实验疫苗组和铝胶疫苗组小鼠对大肠杆菌强毒株C83902均有较好的免疫保护效果,与对照PBS组有明显差异。该研究为进一步提高STI的免疫原性提供了依据。
关键词(KeyWords)： 大肠杆菌;VP4;STI;疫苗
基金项目(Foundation): 新疆生产建设兵团博士基金(兵博02,NKBOISHZO8)
作者(Author): 武军元;陈创夫;王智超;李有文;
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DOI:
参考文献(References)：
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