曹访;杨志红;韩志萍;杨倩;费佳玲;

• 1:湖州师范学院

摘要(Abstract)：

[目的]构建融合表达PPK和绿色荧光蛋白的融合表达载体pCAMBIA1302-PPK。[方法]根据GenBank中登录的大肠杆菌PPK基因序列(L03719)设计引物,以E.coli DH5α基因组DNA为模板,通过PCR扩增得PPK基因,然后用In-Fusion@HD Cloning Kit将PPK基因克隆到pCAMBIA1302载体的Nco I酶切位点。[结果]序列测定结果显示,pCAMBIA1302-PPK含有约2.0 kb的PPK基因片段,说明PPK基因已插入植物表达载体pCAMBIA1302的绿色荧光蛋白基因前。[结论]成功构建了融合表达PPK和绿色荧光蛋白的融合表达载体pCAMBIA1302-PPK。

关键词(KeyWords)： 大肠杆菌;多聚磷酸盐激酶基因;植物表达载体;构建

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(31070451);; 浙江省钱江人才计划项目(2009R10016);; 浙江省自然科学基金(Y5110057)

作者(Author): 曹访;杨志红;韩志萍;杨倩;费佳玲;

Email:

DOI: 10.13989/j.cnki.0517-6611.2012.31.003

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