侯晨;王晓敏;马元媛;毕玉蓉;

• 1:兰州大学生命科学院

摘要(Abstract)：

[目的]探讨芦苇愈伤组织对盐胁迫的生理响应。[方法]以2种芦苇沙芦和水芦愈伤组织为试材,将其置于添加不同浓度NaCl的培养基进行胁迫处理,13 d后测定各项生理指标。[结果]50 mmol/L NaCl胁迫下水芦愈伤组织的细胞活力、TBARS以及活性氧含量均明显增加,而沙芦愈伤组织的则比对照略有降低;300 mmol/L NaCl胁迫下2种芦苇愈伤组织中TBARS与活性氧含量均有所增加;50和300mmol/L NaCl处理下水芦愈伤组织内脯氨酸含量增加,而沙芦愈伤组织的虽在50 mmol/L NaCl处理下降低,但在300 mmol/L NaCl处理下则远远高于水芦愈伤组织的;300 mmol/L NaCl处理下沙芦愈伤组织的4种抗氧化酶活性均显著升高,而水芦愈伤组织的SOD和POD活性虽有所增加,但增幅不如沙芦愈伤组织的。[结论]沙芦愈伤组织具有更强的耐盐性。

关键词(KeyWords)： 盐胁迫;沙芦;水芦;脯氨酸;抗氧化酶

Abstract:

Keywords:

基金项目(Foundation): 高等学校博士学科点专项基金(20050730017);; 甘肃省自然基金(3ZS051-A25-018)

作者(Author): 侯晨;王晓敏;马元媛;毕玉蓉;

Email:

DOI: 10.13989/j.cnki.0517-6611.2008.22.063

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