HPLC法测定榛子雄花中槲皮素和山萘素的含量Determination the Content of Quercetin and Kaempferol in Male Flower of Corylus heterophylla Fisch.
徐建;王婷婷;杨波;张显涛;
摘要(Abstract)：
[目的]建立榛子雄花中槲皮素和山萘素含量的测定方法。[方法]采用高效液相色谱法测定榛子雄花中槲皮素和山萘素的含量。色谱条件:色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm);无水甲醇-浓度0.4%磷酸溶液(50∶50,V/V)为流动相,流速为1.0ml/min,检测波长为360 nm。[结果]槲皮素在0.022～0.658μg/ml浓度范围内线性关系良好,相关系数r=0.999 9,平均回收率为99.4%,RSD为0.73%;山萘素在0.012 1～0.362 0μg/ml浓度范围内线性关系良好,相关系数r=0.999 9,平均回收率为100.4%,RSD为1.49%;测得各地样品中榛子雄花中槲皮素的含量为0.35～0.58 mg/g,山奈素的含量为0.174～0.512 mg/g。[结论]该方法简单、快速、重现性好,可用于榛子雄花中槲皮素和山萘素的含量测定。
关键词(KeyWords)： 高效液相色谱法;榛子雄花;槲皮素;山萘素;含量测定
基金项目(Foundation):
作者(Author): 徐建;王婷婷;杨波;张显涛;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.05.105
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