HPLC法测定榛子雄花中槲皮素和山萘素的含量Determination the Content of Quercetin and Kaempferol in Male Flower of Corylus heterophylla Fisch.
徐建;王婷婷;杨波;张显涛;
摘要(Abstract):
[目的]建立榛子雄花中槲皮素和山萘素含量的测定方法。[方法]采用高效液相色谱法测定榛子雄花中槲皮素和山萘素的含量。色谱条件:色谱柱为Diamonsil C18(250 mm×4.6 mm,5μm);无水甲醇-浓度0.4%磷酸溶液(50∶50,V/V)为流动相,流速为1.0ml/min,检测波长为360 nm。[结果]槲皮素在0.022~0.658μg/ml浓度范围内线性关系良好,相关系数r=0.999 9,平均回收率为99.4%,RSD为0.73%;山萘素在0.012 1~0.362 0μg/ml浓度范围内线性关系良好,相关系数r=0.999 9,平均回收率为100.4%,RSD为1.49%;测得各地样品中榛子雄花中槲皮素的含量为0.35~0.58 mg/g,山奈素的含量为0.174~0.512 mg/g。[结论]该方法简单、快速、重现性好,可用于榛子雄花中槲皮素和山萘素的含量测定。
关键词(KeyWords): 高效液相色谱法;榛子雄花;槲皮素;山萘素;含量测定
基金项目(Foundation):
作者(Author): 徐建;王婷婷;杨波;张显涛;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.05.105
参考文献(References):
- [1]李万林,严仲铠.中国长白山药用植物彩色图志[M].北京:人民卫生出版社,1997:117.
- [2]高国粉,南劲松,田晓乐,等.榛花总黄酮的提取及抗氧化活性的研究[J].中华中医药杂志,2007,22(8):533-534.
- [3]宋玉乔,姚凌云,曹蔚,等.槲皮素的药理作用研究近况[J].西北药学杂志,2002,17(1):40-42.
- [4]冯小龙.银杏叶总黄酮抗肿瘤药效物质基础与药代动力学研究[D].石家庄:河北医科大学,2006.
- [5]GANSAN S,FARIS A N,COMSTOCK A T,et al.Quercetin prevents pro-gression of disease in elastase/LPS-exposed mice by negatively regulatingMMP expression[J].Respir Res,2010,11(1):131.
- [6]AALINKEEL R,BINDUKUMAR B,REYNOLDS J L,et al.The Dietary Bi-oflavonoid,Quercetin,Selectively Induces Apoptosis of Prostate CancerCells by Down-Regulating the Expression of Heat Shock Protein 90[J].Prostate,2008,68(16):1773-1789.
- [7]LAM T K,ROTUNNO M,LUBIN J H,et al.Dietary quercetin,quercetin-gene interaction,metabolic gene expression in lung tissue and lung cancerrisk[J].Carcinogenesis,2010,31(4):634-642.
- [8]ZHANG Y Q,CHEN A Y,LI M,et al.Ginkgo Biloba Extract KaempferolInhibits Cell Proliferation and Induces Apoptosis in Pancreatic CancerCells[J].J Surg Res,2008,148(1):17-23.
- [9]HMLINEN M,NIEMINEN R,VUORELA P,et al.Anti-InflammatoryEffects of Flavonoids:Genistein,Kaempferol,Quercetin,and Daidzein In-hibit STAT-1 and NF-B Activations,Whereas Flavone,Isorhamnetin,Narin-genin,and Pelargonidin Inhibit only NF-B Activation along with Their In-hibitory Effect on iNOS Expression and NO Production in Activated Macro-phages[J].Mediators Inflamm,2007,2007:45673.
- [10]王英辉.HPLC法测定槲寄生胶囊中总黄酮醇苷的含量[J].海峡药学,2007,19(7):48-50.
- [11]邓丹丹,赵浩如,黄罗生.水解时间对银杏黄酮含量的影响[J].中成药,2004,26(8):662-664.
- [12]王聪庆,陈娟,师彦平,等.高效液相色谱法测定前列泰片中山萘素的含量[J].分析测试技术与仪器,2008,14(1):50-53.