邝筱珊;胡松楠;游淑珠;成晓维;王小玉;冯家望;

• 1:珠海出入境检验检疫局技术中心

摘要(Abstract)：

[目的]建立FMV35S基因启动子环介导等温扩增的检测方法。[方法]建立一种检测食品和饲料中FMV35S转基因成分的环介导恒温扩增(LAMP)检测方法,并利用实时浊度法对该LAMP检测方法进行灵敏度、特异性和稳定性评价。[结果]该方法的检测限为0.5%,特异性和稳定性良好。采用LAMP法与荧光PCR方法进行了实际样品对比检测,结果一致可靠。[结论]可采用LAMP法对食品和饲料中FMV35S转基因成分进行检测。

关键词(KeyWords)： 食品;饲料;FMV35S基因;环介导恒温扩增;检测

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 邝筱珊;胡松楠;游淑珠;成晓维;王小玉;冯家望;

Email:

DOI: 10.13989/j.cnki.0517-6611.2014.16.004

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