大豆疫霉菌PsSed5编码基因转录分析及功能研究Transcriptional Analysis and Functional Study of PsSed5 Gene of Phytopnthora sojae
赵伟;迟元凯;汪涛;陈晴晴;Ei Phyu Kyaw;戚仁德;
摘要(Abstract)：
[目的]研究大豆疫霉菌(Phytophthora sojae)SNARE家族之一的编码基因Ps Sed5转录及功能。[方法]利用实时定量PCR分析Ps Sed5在大豆疫霉不同生活史及侵染时间段的转录,利用PEG介导的原生质体稳定转化法,将该基因沉默后分析沉默转化子的表型。[结果]Ps Sed5是组成性表达,在侵染早期表达量较高,Ps Sed5的2个沉默转化子菌丝生长减慢,致病力减弱。[结论]Ps Sed5在大豆疫霉的生长发育及致病性方面都发挥着重要的调控作用。
关键词(KeyWords)： 大豆疫霉;SNARE;PsSed5;基因沉默
基金项目(Foundation): 安徽省自然科学基金项目(1508085MC43)
作者(Author): 赵伟;迟元凯;汪涛;陈晴晴;Ei Phyu Kyaw;戚仁德;
Email:
DOI: 10.13989/j.cnki.0517-6611.2018.08.030
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