一株生防内生真菌D202对立枯丝核菌的抑制作用及生理学特性研究Inhibiting Effect of Dark Septate Endophyte Fungi D202 on Rhizoctonia solani and Its Physiological Characteristics
宋小双;邓勋;遇文婧;尹大川;宋瑞清;
摘要(Abstract):
[目的]为评价内生真菌D202的生防价值以及生理学特性。[方法]通过对峙培养和非挥发性代谢产物抑菌实验,测定生防内生真菌D202对立枯丝核菌(Rhizoctonia solani)的抑制作用,并且对其生理学学特性进行了研究。[结果]拮抗试验中D202对丝核菌的抑制率为67.25%,相对抑制效果为3.54,其非挥发性代谢产物对丝核菌的抑制率为72.35%,菌株D202及其代谢产物可有效抑制立枯丝核菌的生长;内生菌株D202生长最适碳源为葡萄糖,氮源为蛋白胨,在温度为25℃、培养基pH为7时菌落生长最好。[结论]筛选得到的生防内生菌D202具有开发生物源杀菌剂的潜力。
关键词(KeyWords): 深色有隔内生真菌;生防内生菌;立枯丝核菌;抑制作用;生物学特性
基金项目(Foundation): 黑龙江省森工总局项目(SGZJY2010014,SGZJY2012016);; 国家自然科学基金项目(31170597,31200484);; 国家“十二五”科技攻关项目专题(2012BAD19B0801)
作者(Author): 宋小双;邓勋;遇文婧;尹大川;宋瑞清;
Email:
DOI: 10.13989/j.cnki.0517-6611.2014.27.007
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