类黄酮异戊烯转移酶基因在酿酒酵母中的表达Expression Analysis of Flavonoid Prenyltransferase-like Genes in Saccharomyces Cerevisia
袁田田;高娟;刘小烛;
摘要(Abstract):
[目的]对类黄酮异戊烯转移酶基因在酿酒酵母中的表达进行研究。[方法]以粗毛淫羊藿为材料,对其类黄酮异戊烯转移酶基因(EaPT1)进行克隆,并构建重组质粒,将其转化至酿酒酵母INVSc1中表达,进行黄酮类底物粗酶检测。[结果]获得了大小约1 200 bp的EaPT1基因;经SDS-PAGE检测,重组质粒转化后的酵母菌有疑似EaPT1的表达产物;粗酶检测中未能检测到EaPT1酶活性。[结论]为粗毛淫羊藿植物次生代谢,特别是类黄酮生物合成途径的解析奠定基础。
关键词(KeyWords): 淫羊藿苷;重组质粒;异戊烯转移酶;酿酒酵母
基金项目(Foundation): 云南省高等学校本科实验教学示范中心“林木生物技术实验中心”、“生物学”云南省高校优势重点学科及西南林业大学“大型仪器设备共享平台”资助
作者(Author): 袁田田;高娟;刘小烛;
Email:
DOI: 10.13989/j.cnki.0517-6611.2012.18.085
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